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. 2016 Apr 14;291(23):12145–12160. doi: 10.1074/jbc.M115.705368

FIGURE 9.

FIGURE 9.

Phospho-YB-1 regulates FSH- and ERK-dependent target gene mRNA expression. A, GCs were transduced with Ad-YB-1(S102D)-FLAG or Ad-Empty (E) overnight. After media replacement and >1 h of recovery, cells were treated without (veh) or with FSH for 48 h. GCs were collected and analyzed as described in Fig. 8. Quantification of results represents the mean ± S.E. of three independent experiments with (*) representing p < 0.05 and (**) representing p < 0.01, based on a one-tailed Student's t test. B, GCs were transduced with Ad-YB-1(S102A)-FLAG or Ad-Empty overnight. After media replacement and >1 h recovery, cells were treated without (veh) or with FSH for 48 h. Cells were collected and analyzed as described in A. Quantification of results represents the mean ± S.E. of three independent experiments for Egfr, Cyp19a1, Inha Cyp11a1, and Hsd17b1 and the mean ± S.E. of two independent experiments for Lhcgr and Pappa, with (**) representing p < 0.01 and (***) representing p < 0.001, based on a one-tailed Student's t test.