TABLE 3.
mga2Δ transcriptional targets hypoxic microarray
| Functional category and systematic IDa | Common name | S. cerevisiae homologb | Productb | log2 fold change,c WT/mga2Δ | Sre1 targetd |
|---|---|---|---|---|---|
| Lipid metabolism | |||||
| SPAC1B3.16c | vht1 | VHT1 | Biotin transporter | 2.69 | − |
| SPBC18H10.02 | lcf1 | FAA1,FAA3,FAA4 | Long-chain fatty-acid-CoA ligase | 2.66 | − |
| SPCC1450.16c | ptl1 | TGL3 | Triacylglycerol lipase | 2.57 | − |
| SPAC1786.01c | ptl2 | TGL4,TGL5 | Triacylglycerol lipase | 2.32 | − |
| SPAC30D11.11 | IZH3 | Hemolysin-III family protein (homolog to adipo-Q receptor) | 2.31 | − | |
| SPAC589.09 | csr101 | CSR1 | sec14 cytosolic factor family, glycerophospholipid transfer protein | 1.69 | + |
| SPBP4H10.11c | lcf2 | FAA1,FAA3,FAA4 | Long-chain fatty acid-CoA ligase | 1.79 | + |
| SPCC1281.06c | ole1 | OLE1 | Acyl-CoA desaturase, ole1 | 1.74 | − |
| SPCC1235.02 | bio2 | BIO2 | Biotin synthase | 1.68 | − |
| SPAC2F3.09 | hem1 | HEM1 | 5-Aminolevulinate synthase | 1.63 | − |
| SPAC4A8.10 | rog1 | ROG1 | Acylglycerol lipase | 1.62 | − |
| SPAC22A12.06c | fsh2 | FSH2 | Serine hydrolase-like protein | 1.61 | + |
| SPBC646.07c | tsc13 | TSC13 | Enoyl reductase (predicted) | 1.60 | − |
| SPAC56E4.04c | cut6 | ACC1,HFA1 | Acetyl-CoA/biotin carboxylase | 1.58 | − |
| Other | |||||
| SPBC1778.04 | spo6 | DBF4 | Spo4-Spo6 kinase complex regulatory subunit | 3.19 | − |
| SPBC359.04c | pfl7 | Cell surface glycoprotein, DIPSY family | 3.05 | − | |
| SPAC3G6.05 | YOR292C | Mpv17/PMP22 family protein 1 | 2.27 | − | |
| SPBC359.02 | alr2 | Alanine racemase | 2.18 | − | |
| Unknown | |||||
| SPAC17A2.02c | TDA4 | DUF887 family protein | 2.01 | − | |
| SPAC5H10.07 | Sequence orphan | 1.54 | − | ||
| SPCP20C8.03 | Pseudogene | 1.51 | − | ||
| SPCC1281.08 | wtf11 | wtf element Wtf11 | 1.50 | − | |
a Genes showing anaerobic induction in Todd et al. (9) are underlined.
b Descriptions and homologs were obtained from PomBase with some additional hand editing.
c Data are presented as the average changes in expression of genes in sre1Δ (WT) samples after 1.5 h without O2 compared with the expression of genes in sre1Δmga2Δ (mga2Δ) samples after 1.5-h without O2. For clarity, only data greater than 2 S.D. from the average fold change are presented in this table. Full results are detailed in supplemental Tables S4 and S5.
d Statistically significant target assignment from low oxygen microarray was from Todd et al. (9).