FIGURE 5.

Binding of Htm1 to Pdi1 is required for ERAD. A, Pdi-HA was precipitated under non-denaturing conditions from cell lysates of yeast strains expressing Htm1-Myc or the indicated truncated variants. The proteins were detected by immunoblotting using the given antibodies. B and C, degradation of CPY* (B) or Erg3-Myc (C) was analyzed by pulse-chase experiments in the given yeast strains. Graphs represent the mean values of at least three independent experiments (CPY* and Erg3-Myc in Δhtm1 is n = 2), and the S.D. is given.