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. 2016 Apr 18;291(23):12245–12253. doi: 10.1074/jbc.M116.722264

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Ltn1 is also essential to prevent the accumulation of aberrant protein aggregates. Polysome extracts from rqc1Δ, ltn1Δ, and rqc1Δltn1Δ cells expressing the TAP-NonStop reporter were prepared and separated on a sucrose gradient (10–50%), and TCA-precipitated fractions were migrated on polyacrylamide gels and analyzed by Western blotting using antibodies against the TAP-NonStop (PAP) and Nog1 as a loading control and a marker of the 60S-sedimenting fractions. Aggregated species of the TAP-NonStop accumulating in the stacking gel are annotated. NS, NonStop.