FIGURE 4.
Secretion of furin-cleaved fHjv, A183R-fHjv, and G313V-fHjv from liver into the circulation. Eight-week-old male Hjv−/− mice were intraperitoneally injected with AAV8-fHjv, A183R-fHjv, or G313V-fHjv at ∼5 × 1011 (+) and ∼1013 (++) genome particles per mouse as described in the legend to Fig. 2. A and B, Western blotting analysis of fHjv and β-actin in 150 μg of liver extracts and soluble fHjv in 60 μl of serum. Two images for both liver extract and serum fHjv with different exposures were presented in B. C, quantification of serum fHjv bands in Western blot images by ImageJ software (n = 5).