FIGURE 1.
Construction of pHluorin-Myc-tagged GABAAR α2 mouse. A, schematic representation of pHluorin-Myc tagged at the N terminus of the GABAAR α2 subunit. B, illustrations of the targeting vector and the targeted α2 subunit gene with addition of pHluorin-myc into exon 3. C, genotyping for wild type (−/−), heterozygotes (+/−), and pHα2 (+/+) mice using primers flanking pHluorin. D, cresyl violet staining of hippocampus shows there are no gross morphological changes in the hippocampal anatomy of pHα2 mice. Scale bar, 500 μm. E, DNA and protein sequence of N-terminal segment of pHα2 knock-in mouse. pHluorin (green, italics) and Myc (red, underline) reporters are depicted.