FIGURE 2.
Characterization of pHα2 mice. A, representative Western blots of hippocampal lysates from WT and pHα2 mice. The pHluorin-Myc tag increases the molecular weight of the GABAAR α2 subunit. B, pooled quantification of protein expression shows there are no significant differences in the total expression levels of GABAAR α4 (p = 0.80, t test, n = 5), β3 (p = 0.78, t test, n = 5), GAPDH (p = 0.99, t test, n = 4), gephyrin (p = 0.46, t test, n = 5), NMDAR NR1 (p = 0.09, t test, n = 5), and tubulin (Tub) (p = 0.99, t test, n = 4) between the two genotypes. Data represent means ± S.E.