FIGURE 5.

Two-step purification to isolate pHα2 complexes. Detergent-solubilized hippocampal and cortical lysates of age- and sex-matched WT and pHα2 mice were immunoprecipitated with Myc followed by GFP-Trap and subjected to SDS-PAGE and silver staining (A). Representative silver-stained gel depicts bands of interest (arrow) that were excised from pHα2 and the corresponding WT lane for mass spectrometry analysis. Protein coverage of GABA_AR α2 subunit (blue, underline) identified by MS analysis (B). Example of MS/MS spectrum for tryptic peptide identified as GABA_AR α2 is shown (C). The sequence of the identified peptide is indicated.