FIGURE 6.

Single-step purification to isolate pHα2 complexes. Detergent-solubilized hippocampal and cortical lysates from WT and pHα2 mice were immunoprecipitated with GFP antibodies and subjected to SDS-PAGE and colloidal Coomassie staining (A). Each gel lane was cut into five pieces and pooled for mass spectrometry analysis. Protein coverage of GABA_AR α2 subunit (blue, underline) identified by MS analysis (B). Example of MS/MS spectrum for tryptic peptide identified as GABA_AR α2 is shown (C). The sequence of the identified peptide is indicated.