Ephexin, KCTD12, and mGluR5 bind pHα2.
A, hippocampal and cortical lysates from WT and pHα2 mice immunoprecipitated with GFP-Trap beads. Bound proteins were immunoblotted with mGluR5, GFP, ephexin, β3, and KCTD12 antibodies. B and C, transfection of HEK293 cells with a combination of plasmids encoding pHα2, β3, FLAG-ephexin, and empty vector. Cell lysates were immunoprecipitated with FLAG (B) or GFP (C) and bound proteins were detected by Western blotting.