TABLE 4.
Regulators of protein trafficking, stability, and cytoskeletal targeting associated with pHα2 identified using GFP-Trap purification
| Gene symbol | Reference | Name | Total peptide |
|
|---|---|---|---|---|
| WT | pHα2 | |||
| Adam22 | ADA22_MOUSE | ADAM metallopeptidase domain 22 | 0 | 9 |
| Add3 | ADDG_MOUSE | Adducin 3 | 4 | 17 |
| Afg3l2 | AFG32_MOUSE | AFG3-like AAA ATPase 2 | 8 | 42 |
| Cul1 | CUL1_MOUSE | Cullin 1 | 2 | 22 |
| Cul2 | CUL2_MOUSE | Cullin 2 | 3 | 17 |
| Cul3 | CUL3_MOUSE | Cullin 3 | 5 | 21 |
| Dcaf8 | DCAF8_MOUSE | DDB1- and CUL4-associated factor 8 | 0 | 7 |
| Ddb1 | DDB1_MOUSE | Damage-specific DNA-binding protein 1 | 1 | 10 |
| Dnaja1 | DNJA1_MOUSE | DnaJ heat shock protein family (Hsp40) member A1 | 2 | 11 |
| Dync1i2 | DC1I2_MOUSE | Dynein, cytoplasmic 1, intermediate chain 2 | 3 | 13 |
| Epn1 | EPN1_MOUSE | Epsin 1 | 0 | 8 |
| Erlin1 | ERLN1_MOUSE | Endoplasmic reticulum lipid raft-associated 1 | 3 | 13 |
| Exoc3 | EXOC3_MOUSE | Exocyst complex component 3 | 1 | 18 |
| Exoc7 | EXOC7_MOUSE | Exocyst complex component 7 | 1 | 28 |
| Exoc8 | EXOC8_MOUSE | Exocyst complex component 8 | 2 | 13 |
| Hook3 | HOOK3_MOUSE | Hook microtubule-tethering protein 3 | 1 | 8 |
| Ipo9 | IPO9_MOUSE | Importin 9 | 2 | 10 |
| Kbtbd7 | G5E8C2_MOUSE | Kelch repeat and BTB (POZ) domain containing 7 | 0 | 13 |
| Kif3a | KIF3A_MOUSE | Kinesin family member 3A | 7 | 26 |
| Lrrc7 | LRRC7_MOUSE | Leucine-rich repeat containing 7 | 1 | 7 |
| Magi3 | MAGI3_MOUSE | Membrane-associated guanylate kinase, WW, and PDZ domain containing 3 | 0 | 14 |
| Mapre2 | MARE2_MOUSE | Microtubule-associated protein RP/EB family member 2 | 0 | 10 |
| Napa | SNAA_MOUSE | NSF attachment protein α | 5 | 22 |
| Napb | SNAB_MOUSE | NSF attachment protein β | 2 | 15 |
| Nefl | NFL_MOUSE | Neurofilament, light polypeptide | 3 | 11 |
| Ngly1 | NGLY1_MOUSE | N-Glycanase 1 | 0 | 10 |
| Os9 | OS9_MOUSE | Osteosarcoma-amplified 9, endoplasmic reticulum lectin | 0 | 8 |
| Psmd9 | PSMD9_MOUSE | Proteasome 26S subunit, non-ATPase 9 | 2 | 9 |
| Scamp3 | SCAM3_MOUSE | Secretory carrier membrane protein 3 | 0 | 8 |
| Sec23b | SC23B_MOUSE | Sec23 homolog B, COPII coat complex component | 0 | 9 |
| Sqstm1 | SQSTM_MOUSE | Sequestosome 1 | 1 | 8 |
| Sv2a | SV2A_MOUSE | Synaptic vesicle glycoprotein 2A | 15 | 64 |
| Sv2b | SV2B_MOUSE | Synaptic vesicle glycoprotein 2B | 6 | 35 |
| Trim32 | TRI32_MOUSE | Tripartite motif containing 32 | 7 | 24 |
| Uchl1 | UCHL1_MOUSE | Ubiquitin C-terminal hydrolase L1 | 7 | 22 |
| Usp9x | USP9X_MOUSE | Ubiquitin-specific peptidase 9, X-linked | 2 | 12 |
| Vps35 | VPS35_MOUSE | VPS35 retromer complex component | 21 | 69 |
| Vps52 | VPS52_MOUSE | VPS52 GARP complex subunit | 2 | 21 |