Fig 5. Differentially methylated genes between Nasonia vitripennis (Nv) and Nasonia giraulti (Ng) display a bias toward methylated alleles for both total and allelic expression levels.

(A) Boxplot of parental Nv/Ng expression ratios for differentially methylated genes. Criteria are provided in materials and methods. Differentially expressed genes (FDR < 0.05) are shown in red. Among these genes, the methylated species show significantly higher expression compared to the species with the non-methylated allele. (B) Boxplot of F1 allele-specific expression ratios for differentially methylated genes between Nv and Ng, quantified by Nv allelic expression divided by Ng allelic expression in F1s (x-axis). Criteria are provided in materials and methods. Genes that are monoallelically expressed in Nv (Ng) were plotted at the right (left), because their log₂ value is undefined. Genes with significant allelic expression bias (FDR < 0.01) are shown in red, and their methylation patterns are significantly biased toward the highly expressed alleles. Data presented in this figure can be found at http://dx.doi.org/10.5061/dryad.qf2t8.