Fig 1. Identification of Dpp target genes.

(A) Race expression in dorsal and lateral views of wildtype and Tkv^QD over-expressing embryos at the onset of gastrulation. (B) RNA in situ hybridization staining of wildtype embryos with antisense probes to detect transcripts identified as being positively regulated by Dpp signaling, based on the RNA-seq analysis. Dorsal and lateral views of stage 6 and 9 embryos, respectively, are shown. (C) As in (B), except stage 6 and 11 embryos are shown. (D) Expression of selected transcripts in embryos lacking dpp or carrying 4 copies of dpp, compared to the wildtype pattern.