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. 2016 Jul 5;12(7):e1006164. doi: 10.1371/journal.pgen.1006164

Fig 3. Dpp-EGF signaling cross-talk in the embryo.

Fig 3

(A) RNA in situ hybridization showing expression of the peak Dpp target genes Race, hnt and ush in stage 6 wildtype and kek1 mutant embryos (dorsal views). (B) Visualization of Race mRNA by fluorescent in situ hybridization (red) and dpERK protein (green) in wildtype and kek1 mutant embryos. Top row shows the merge image with DAPI (blue), lower panels show the separate channels for Race and dpERK. (C) As in (B) except that kek1 and wildtype embryos are stained for Race mRNA and pMad protein at stages 5 and 6, as labelled. (D) RNA in situ hybridizations showing egr mRNA in stage 5 and 6 wildtype and kek1 mutant embryos. (E) Graph showing the expression widths of the Dpp target genes in wildtype and kek1 mutant embryos. Error bars are SEM, n≥10 across different biological repeats, *P<0.0001, two-way ANOVA.