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. 2016 Jul 5;12(7):e1006164. doi: 10.1371/journal.pgen.1006164

Fig 4. Negative regulation of the Dpp pathway by EGF signaling.

Fig 4

(A) Visualization of Race and hnt mRNA in stage 6 wildtype and egfr mutant embryos by RNA in situ hybridization. (B) Detection of Race mRNA by fluorescent RNA in situ hybridization (red) and dpERK protein (green) by immunostaining in wildtype and egfr mutant embryos (late stage 6, dorsolateral views). (C) As in (B) except that rho mutant embryos are compared to wildtype, embryos are at early stage 6 and shown as dorsal views. (D) Graph showing the expression widths of the Dpp target genes in wildtype embryos and the mutants tested in (A)-(C). Error bars are SEM, n≥10 across different biological repeats, *P<0.0001, ordinary one-way ANOVA.