Table 1. Sequences of primers used in this study.
| Primer | Sequence (5ˊ-3ˊ) | Target region | Amplicon (bp) | Reference |
|---|---|---|---|---|
| Sad l-F | CCAAAGGAGGTGCCTGTTCA | Cotton endogene | 107 | [25] |
| Sad I-R | TTGAGGTGAGTCAGAATGTTGTTC | |||
| G2-F-1 | GGCTCCAAATCCATTACCAACC | G2-aroA | 896 | This work |
| G2-R-2 | CGCAGGTTCGCCAGTTCA | |||
| Kana-F1 | AACACGGCGGCATCAGAGCA | Special primers for Tail-PCR | ||
| Kana-F2 | TACCGAGGGGAATTTATGGAACG | |||
| Kana-F3 | GTTGCGGTTCTGTCAGTTCCAA | |||
| F1 | CTGGCGTAATAGCGAAGAG | Flanking primer of the 3ˊ-terminus | <927 | |
| R1 | TTGAAAGACAAGGGATGGA | |||
| F2 | CTGGCGTAATAGCGAAGAG | <1770 | ||
| R2 | AAAGAACTAAACTGGAAACCC | |||
| F3 | CCAGCGAGACGAGCAAGA | <848 | ||
| R3 | ACAAGCGGAGGCGGTATT | |||
| F4 | GCAGGAACGCAAACATTG | <1942 | ||
| R4 | AAAGAACTAAACTGGAAACCC | |||
| F5 | AGCCCGATGGCTACTAAG | <1945 | ||
| R5 | TTGAAAGACAAGGGATGGA | |||
| 5ˊ-F1 | TGTGGGCCATCGCCCTGATA | Upstream screening primers of the 5ˊ-terminus | ||
| 5ˊ-F2 | GTTGCGGTTCTGTCAGTTCCAA | |||
| 5ˊ-F3 | GTCATAACGTGACTCCCTTAATTCTCC | |||
| 5ˊ-R1 | CCTATTACACGGCTATGC | Downstream screening primers of the 5ˊ-terminus | ||
| 5ˊ-R2 | CTCAATCAGCCCCAAAAT | |||
| 5ˊ-R3 | CCACCCATTCCCCAAAGT | |||
| 3ˊ-F1 | GCTCCTTTCGCTTTCTTCCC | Upstream screening primers of the 3ˊ-terminus | ||
| 3ˊ-F2 | TCGCTTTCTTCCCTTCCTTT | |||
| 3ˊ-F4 | AGGGTTCCGATTTAGTGCTT | |||
| 3ˊ-F6 | TCCTTTCGCTTTCTTCCCTT | |||
| 3ˊ-F10 | CTAAAAGGCAGGAACGCAAA | |||
| 3ˊ-R1 | GGTGCATTAAAAGGGTGGCA | Downstream screening primers of the 3ˊ-terminus | ||
| 3ˊ-R2 | GTGCATTAAAAGGGTGGCAT | |||
| 3ˊ-R3 | TCTGACATCATGGATCGCAA | |||
| 3ˊ-R4 | GGCCTCAATTCTCTCCATCA | |||
| 3ˊ-R6 | CATCAGCACTTTCGAATGCA | |||
| 3ˊ-R7 | CGTTAACTTCACCGCTCATC | |||
| 3ˊ-R8 | ACAAGGGATGGACTGTCTTC | |||
| 3ˊ-R9 | ACATGGCGTCTTCTTCATCT | |||
| 3ˊ-R12 | ACACTTACATGGCGTCTTCT |