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. 2016 Jul 5;11(7):e0158694. doi: 10.1371/journal.pone.0158694

Fig 6. Effect of 20 μM 1j and 25 μM CDDP on ROS production in the cancerous and normal cells.

Fig 6

a-b. DCFH-DA staining shows increased ROS production upon exposure of both the compounds in CCF-4 and NKE cells. c-d. Differential induction of ROS on CCF-4 and NKE cells upon exposure to 20 μM 1j and 5mM NAC. e. Intracellular ROS production was detected by changes in the fluorescence intensity of DCF by fluoroscent microscopy (20X). Data are representative of three independent experiments. f. Effect on cell viability of CCF-4 cells upon exposure to 20 μM 1j and 5mM NAC. Each column represents mean ±SD, n = 6. ‘‘*” represents the significant difference between the normal control and 1j treated cells (P* < 0.05).