Figure 3.

Effect of delphinidin on the expression of β-catenin destruction complex in PC3. Cells were treated with specified concentrations of delphinidin for 72 hours and harvested, after which total cell lysates were prepared. Protein levels were evaluated by Western blotting for casein kinase 1 (CK1), protein phosphatase 2A (PP2A), glycogen synthase kinase 3 (GSK3), Axin, and adenomatous polyposis coli (APC). Equal loading of proteins was confirmed by stripping the immunoblot and reprobing it for β-actin. The immunoblots shown here are representative of three independent experiments.