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. 2016 Apr 12;291(24):12761–12770. doi: 10.1074/jbc.M115.704627

FIGURE 5.

FIGURE 5.

HDAC8 inhibition does not affect ER binding at candidate genes in MCF7 cells. Estrogen-deprived MCF7 cells were transfected with control/RAD21 siRNA or treated with 25 μm of PCI-34051 for 48 h and then treated with vehicle or 100 nm of estradiol for 45 min. Schematics of the SOX4 gene (A) and IL20 gene (B) showing RAD21, estradiol-induced RAD21, and ER binding, mapped on the UCSC genome browser (2006 NCBI36/hg18 version) using previously published ChIP-seq data in MCF7 cells. Numbers represent positions of the ChIP primers used to amplify various sites. Bar plots represent qChIP analyses at SOX4 gene (C) and at IL20 gene (D) using RAD21 and ER antibodies in RAD21 siRNA or PCI-34051-treated MCF7 cells. Binding is shown relative to input chromatin. Error bars are ±S.E. of four/three independent biological replicates for the knockdown and PCI-34051 experiments, respectively. Significance was determined using two-way ANOVA. *, p ≤ 0.05; **, p ≤ 0.01.