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Exterior waist mutants of SsoMCM. A, position of alanine waist mutants (purple, this study; red, K323A/R440A) mapped onto the ARDD map for apo- versus 3′-tail/5′-tail DNA. B, plot of the ATPase rates performed in the absence (blue) and presence (green) of fork DNA. Error bars, S.E. C, conservation of waist mutations (boxed yellow) from S. solfataricus (Sso), Saccharomyces cerevisiae (Sc), Xenopus laevis (Xl), and Homo sapiens (Hs).
