TABLE 1.

Primers used for RT-PCR/qRT-PCR analyses, cDNA cloning, and mutagenesis

Gene	Forward primer (from 5′ to 3′)	Reverse primer (from 5′ to 3′)	Purpose	Species
Tnf-α	tgatcggtccccaaagg	ggtctgggccatagaactga	qRT-PCR	Mouse
Cox-2	tgtacaagcagtggcaaagg	gcagccatttccttctctcc	qRT-PCR	Mouse
Cxcl1	acccaaaccgaagtcatagc	tggggacaccttttagcatc	qRT-PCR	Mouse
Cxcl2	agtttgccttgaccctgaag	ctttggttcttccgttgagg	qRT-PCR	Mouse
Ccl2	ttgtcaccaagctcaagagaga	gaggtggttgtggaaaaggtag	qRT-PCR	Mouse
Cxcr2	atgccctctattctgccagat	gtgctccggttgtataagatgac	qRT-PCR	Mouse
Ccr2	atccacggcatactatcaacatc	caaggctcaccatcatcgtag	qRT-PCR	Mouse
Cd68	cttcacgatgacacctacag	tcttggactagtagcagtgc	qRT-PCR	Mouse
CXCL1	agggaattcaccccaagaac	caccagtgagcttcctcctc	RT-PCR	Human
COX-2	gcaaattgctggcagggttg	agggcttcagcataaagcgt	RT-PCR	Human
CCL20	gcgcaaatccaaaacagact	caagtccagtgaggcacaaa	RT-PCR	Human
PLCϵ	gctcttcagcggattattggaa	tcataccgtccatcctcctgata	RT-PCR	Human
CCL2	ccccagtcacctgctgttat	tggaatcctgaacccacttc	qRT-PCR	Human
CXCL8	cttggcagccttcctgattt	ttctttagcactccttggcaaaa	qRT-PCR	Human
PLCϵ	tccagaagagggatacatgg	tcataccgtccatcctcctgata	qRT-PCR	Human
β-Actin	atgaagatcaagatcattgctcctc	acatctgctggaaggtggacag	RT-PCR/qRT-PCR	Mouse/human
GAPDH	gaaggtgaaggtcggagtc	gaagatggtgatgggatttc	qRT-PCR	Human
PEA15	aaagaattcccgctgagtacgggaccctc	aaaaggtacctcaggccttcttcggtggg	PCR Cloning	Human
PEA15	atccccgccgccaagaagtacaaagacatta	cttggcggcggggatacgggttagcttg	S104A mutant construction	Human
PEA15	atccccgacgccaagaagtacaaagacatta	cttggcgtcggggatacgggttagcttg	S104D mutant construction	Human
PLCϵ	aatgttctcctgtgactcatcattttttgaggcaa	aagctaaaagcccatcagacgccagtggatatctt	ΔX mutant construction	Human