Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 31;291(24):12799–12808. doi: 10.1074/jbc.M116.724245

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — WTIP links nephrin to Lats1 inhibition. A, lysates from HEK293T cells coexpressing CD16/7-nephrinY3F or CD16/7-nephrinY3F-3xSH3 with Lats1-FLAG in the presence or absence of WTIP-HA were stimulated with anti-CD16 antibody, immunoprecipitated (IP) for GFP, and immunoblotted (IB) for GFP, FLAG, and HA. Lats1 binds the CD16/7-nephrinY3F-3xSH3 fusion protein but not CD16/7-nephrinY3F alone upon coexpression of WTIP. B, immunoblot of equivalent lysates from the third and fourth lanes of A with phospho-Lats1 Ser-909 shows a decrease in Lats1 activation upon complex formation between CD16/7-nephrinY3F-3xSH3, WTIP, and Lats1. C, densitometry of B, showing a statistically significant 2.4-fold reduction of Lats1 phosphorylation on Ser-909 coincident with complex formation between CD16/7-nephrinY3F-3xSH3, WTIP, and Lats1 (n = 4). **, p < 0.01.