FIGURE 6.

PS1- and PS2- containing γ-secretase complexes are targeted to distinct cellular compartments. A, COS-7 cells were co-transfected with 4γ-PS/APH1 constructs (the specific PS and APH1 subunits are indicated on the left and top of images, respectively), and the transactivator protein expression plasmid; transgene expression was induced with doxycycline for 2 h the next day, cells were fixed 1 day after induction, and EGFP fluorescence was acquired using wide field epifluorescence microscopy. B–D, primary hippocampal neurons from embryonic (embryonic day 18) rats were transfected with 4γ-PS/APH1 constructs (the variable subunits are indicated on the left (PS) and the top (APH1) of each panel), and the transactivator protein expression plasmid at 6 DIV, induced with doxycycline at 16 DIV for 5 h, and fixed the following day before observation of EGFP signal in the soma (B), the axon (C), and dendrites (D) of transfected neurons using wide field epifluorescence microscopy. Scale bars, 10 μm.