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. 2016 Apr 15;291(24):12838–12850. doi: 10.1074/jbc.M116.722447

FIGURE 3.

FIGURE 3.

Substrate binding and activity of SliLPMO10E. Inset SDS-PAGE to monitor the binding of SliLPMO10E to cellulose (filter paper) or squid-pen β-chitin. Fractions shown are untreated SliLPMO10E, the protein content in the supernatant (unbound) or bound to the substrate (bound). MALDI-TOF analysis of SliLPMO10E products from squid-pen β-chitin. DPna1 = aldonic acid; DPn−2 = lactone or C4 ketoaldose (measured molecular weight). DP4−2 + Na+ (851.3), DP4al + Na+ (869.3), DP4al− + 2Na+ (891.3), DP6−2 + Na+ (1257.5), DP6al + Na+ (1275.5), DP6al− + 2Na+ (1297.5), DP8−2 + Na+ (1663.6), DP8al + Na+ (1681.7), DP8al− + 2Na+ (1703.6).