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Thermodynamics of Cu(II) binding to SliLPMO10E at pH 5 and 25 °C. An ITC thermogram and fit to a sequential binding model (solid line) upon titrating SliLPMO10E with Cu(II). Thermodynamic parameters obtained are reported in Table 5. Inset reports the heat of dilutions from a buffer to buffer and a Cu(II) to buffer titration.
