TABLE 1.
Transcription analysis of the seven lpmo genes in S. lividans
RNA sequence data obtained and reported earlier in liquid minimal media and glucose/mannitol as a carbon source (61) were analyzed with the S. lividans 1326 genome sequence as input (37).
| Gene | Protein | S. lividans 1326a (RPKM)b | S. lividans 1326 + Cua (RPKM)b |
|---|---|---|---|
| SLI0440 | SliLPMO10A | 0 | 0 |
| SLI0614 | SliLPMO10B | 10 | 9 |
| SLI1466 | SliLPMO10C | 0 | 6 |
| SLI2039 | SliLPMO10D | 0 | 2 |
| SLI3182 | SliLPMO10E | 22 | 27 |
| SLI6742 | SliLPMO10F | 0 | 0 |
| SLI7441 | SliLPMO10G | 0 | 3 |
a The transcriptomes of WT S. lividans 1326 were obtained without addition of copper to the growth medium. The copper-induced transcriptome was obtained after a 2-h exposure to 400 μm Cu(II).
b The selected expression measure is the RPKM and is defined as the reads/kb of exon/million mapped reads, i.e. dividing the total number of exon reads (in this case one exon per reference sequence) by the number of mapped reads (in millions) times the exon length (in kb) (62).