TABLE 5.

Kinetic and thermodynamic parameters determined from stopped-flow fluorescence, fluorescence titrations, and ITC at 25 °C for Cu(II) binding to the various forms of SliLPMO10E

^a Experimental errors are reported in parentheses and are the standard errors from triplicate experiments.

^b Data were determined from static fluorescence titration.

^c Data were determined from stopped-flow fluorescence.

^d Experimental errors are reported in parentheses and are the standard errors from triplicate experiments for SliLPMO10E and duplicates for H187A and SliLPMO10E-EXT.

^e Parameters were derived from a sequential binding model.

^f Parameters were derived from a one set of sites binding model.