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. 2016 Apr 8;291(24):12732–12746. doi: 10.1074/jbc.M115.704734

FIGURE 2.

FIGURE 2.

Three-dimensional EM and solution SAXS models of BMP-7 PD-GF complex. Three-dimensional structure of BMP-7 complex was generated using TEM. A (top), representative electron micrograph of BMP-7 complex molecules (scale bar, 100 nm); bottom, 12 images selected from 140 class sum images of 9,000 particles that represent different views of BMP-7 complex (box size = 29.4 × 29.4 nm). B, class sum images were used to generate a three-dimensional TEM model of BMP-7 complex with 2-fold symmetry using angular reconstitution. C, superimposition of the BMP-9 complex structure (33) at 20 Å with the determined BMP-7 EM envelope suggests that the angle between the boomerang arms may be wider in BMP-9.