TABLE 1.
CAV1 and CAV1-associated proteins identified as DCT interactors in MJS lysates using high performance liquid chromatography coupled with tandem mass spectrometry analysis
The Uniprot accession number from the human proteome, protein name, summed sequence coverage, number of unique peptides, and spectral counts in each of the three samples immunoprecipitated with anti-TYR antibody (α-hPep7) (negative control), α-hDCT (anti-hDCT luminal domain, aa 27–439), and α-C terminus (anti-hDCT C-terminus domain, aa 506–519) are given for each protein.
| Uniprot accession no. | Protein name (Alternative name) | Total sequence coverage | Total unique peptides | Spectral counts for IP with α-hPep7 antibody | Spectral counts for IP with α-hDCT antibody | Spectral counts for IP with α-C-terminus antibody | Protein mass |
|---|---|---|---|---|---|---|---|
| % | kDa | ||||||
| P40126 | l-Dopachrome tautomerase (tyrosinase-related protein 2) | 33.53 | 14 | 0 | 26 | 63 | 59.1 |
| Q03135 | Caveolin-1 (CAV1) | 24.16 | 3 | 0 | 6 | 3 | 20.5 |
| Q6NZI2 | Polymerase I and transcript release factor (Cavin-1) | 21.54 | 7 | 0 | 19 | 0 | 43.4 |
| Q969G5 | Protein kinase Cδ-binding protein (Cavin-3) | 30.27 | 7 | 0 | 19 | 0 | 27.7 |