FIGURE 4.

The stp allele exhibits a lesion in cx43. A, meiotic mapping of the stp allele revealed ∼460 kb of contiguous sequence of the stp region. Only cx43 exhibits an ENU-induced lesion. Red lines represent the region spanning the stp critical interval. B, electrophoretograms showing the mutation identified in the coding sequence of the stp allele. C, cartoon of Cx43 protein in a double-lipid bilayer indicating the location of the ENU-induced lesion. D, BLAST search results showing the highly conserved amino acid sequence of Cx43. E, E′, F, and F′, expression of mCherry fluorescent protein in the vertebrae and caudal fin, driven by the cx43 promoter. E and E′, mCherry was expressed at the growing edges of vertebral centrums of 21 dpf fish. F and F′, mCherry was expressed at junctions between fin ray segments of 36 dpf fish. Scale bars, 1000 μm in E and F and 200 μm in E′ and F′.