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. 2016 Jun 7;36(2):676–684. doi: 10.3892/or.2016.4860

Figure 2.

Figure 2

Fluoxetine (FLT) treatment for 24 h increases the early apoptosis rate and induces caspase-3 activation of C6 glioma cells. Cells were incubated with various concentrations of FLT for 24 h. (A and B) Early apoptosis was measured by the Annexin V assay using flow cytometry. (C) The activity of caspase was determined using a selective luminogenic substrate (Ac-LETD-pNA). The data are presented as means ± SEM from at least three independent experiments (*P<0.05, **P<0.01 as compared with the control).