The frequency of circulating Th17, Tc17 and γδT17 cells in all individuals. Peripheral blood mononuclear cells (PBMCs) obtained from patients with lung adenocarcinoma (LA) and healthy controls (HCs) were stained with labeled antibodies and analyzed using flow cytometric analysis. (A) PBMCs were gated initially on living lymphocytes and then on CD3+ T cells. (B) Representative plots of IL-17A+CD4+ T (Th17), IL-17A+CD8+ T (Tc17) and, IL-17A+γδ+ T (γδT17) cells in total CD3+ T cells from HCs. (C) Representative plots of Th17, Tc17, and γδT17 cells in total CD3+ T cells from patients with LA. Comparison of (D) Th17, (E) Tc17, and (F) γδT17 cells presented as percentages of total CD3+ T cells, between patients with LA and HCs. The data shown represent the means ± SEM. *P<0.05, **P<0.01 were considered to represent significant differences compared with HCs.