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. 2016 Jun 30;22:2267–2277. doi: 10.12659/MSM.898702

Figure 2.

Figure 2

Effect of TGF-β1 gene targeting on colony formation, cell survival, and apoptosis. (A) Silencing of TGF-β1 transcripts in positive clones was confirmed by RT-PCR. (B) Silencing of TGF-β1 protein in positive clones was confirmed by Western blot assay. (C) Photomicrographs showing soft-agar colony formation and histogram showing number of colonies formed by 8505C cells transfected with siRNA. (D) 8505C cells were transfected with the siRNA plasmid for 120 h, cell survival rate was detected by MTT assay, 8505C cells were transfected with the siRNA plasmid for 120 h, and cell apoptotic rate was detected by flow cytometry assay. (E) 8505C cells were transfected with the siRNA plasmid for 120 h, and cell apoptotic rate was detected by flow cytometry assay. Vs. control, * p<0.05; ** p<0.01.