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. 2016 May 2;15(4):766–777. doi: 10.1111/acel.12488

Figure 1.

Figure 1

Aging enhances atherosclerosis during HFD. (A–B) Young (5 months of age), middle aged (11 months of age) and aged (15 months of age) male and female Ldlr −/− mice were maintained on a chow diet until 3 months prior to tissue harvest when they were switch to a HFD. At the end of the HFD feeding period, the aortic root was obtained and stained with H&E, and lesion size was enumerated. Representative images of male mice are shown in A, and quantification is shown B. *P < 0.01 (t‐test). (C) As per A, necrotic core lesion assessment based upon the area of acellular staining within the H&E images of the aortic root of the each of the experimental cohorts. *P < 0.01 (t‐test). (D) Representative photographs of the ascending aortic arch of aged or young male Ldlr −/− mice fed a diet for 6 or 12 weeks. Atherosclerotic plaque is seen as white, opaque material. (E) Young (5 months) and aged (15 months) male Ldlr −/− mice were fed a HFD for 3 months prior to tissue harvest and the lesions were stained with alizarin red to assess lesion calcification (red staining). A representative image of young and aged aortic root lesions are shown at 2×. N = 8 biological replicates/group. (F) Inset of the box of aged aortic root shown in G at 20×.