Table 3.
Production of 8-oxo-dG by H2O2 or LOOH in lipid micelles containing dG
| Fe2+ (µmol/L)1 | Components of micelles2 |
|||
|---|---|---|---|---|
| Oleic acid (150 µmol/L) | Linoleic acid (150 µmol/L) | Linoleic acid (145 µmol/L) and LOOH (5.0 µmol/L) | H2O2 (50 µmol/L)1 | |
| Produced number of 8-oxo-dG per 105 dG3 | ||||
| 0 | 0.82 ± 0.15a | 0.47 ± 0.07a | 6.04 ± 0.56b | 2.17 ± 0.36a |
| 0.5 | 0.97 ± 0.18a | 0.70 ± 0.04a | 11.34 ± 0.38c | 6.93 ± 1.66b |
| 10 | — | — | 9.30 ± 2.21d | 9.29 ± 1.26d |
1FeSO4 and H2O2 were added to the liposomal mixture prepared as shown in Materials and Methods. 2Oleic acid, linoleic acid, or a mixture of linoleic acid and LOOH was mixed in a suspension of lysophosphatidylcholine and mono-olein as shown in Materials and Methods. 3Values are mean ± SD (n = 6) and different letters indicate statistically significant differences (p<0.05). The commercial dG originally contained 8-oxo-dG at levels of 0.58 ± 0.08 number per 105 dG, and the present results include the original amounts of 8-oxo-dG.