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. 2016 Jul 6;7:84. doi: 10.3389/fendo.2016.00084

Figure 3.

Figure 3

Hexokinase 2 (HK2) expression and in 2D and 3D cultures of PC3 cells grown alone or in transwell coculture with adipocytes. Expression of HK2 [(A), green] and MitoTracker [(B), red] merged (C) in PC3 cells grown alone (A–C) or in transwell (E–G) in 2D coculture. (D,H) No HK2 primary antibody controls; DAPI was used as nuclear marker (blue). (I,J) 3D reconstruction of CA9 expression in tumor spheroids grown alone (I) or in transwell with adipocytes (J); green arrow (X), red arrow (Y), and blue arrow (Z) indicate orientation of the spheroid in 3D space. HK2 and MitoTracker fluorescence to show HK2 localization to the mitochondria in PC3 cells cultured alone (K–N) or in Transwell with adipocytes (P–S). (O,T) No HK2 primary antibody controls; 40× images; bar, 50 μm.