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. 2016 Jul 6;7:84. doi: 10.3389/fendo.2016.00084

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Copyright © 2016 Herroon, Diedrich and Podgorski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Three-dimensional invasion of tumor cells toward bone marrow adipocytes. (A) Diagram depicting the experimental setup. Tumor cells invaded through collagen I matrix toward bone marrow adipocytes. Adipocytes (BODIPY, green); Tumor cells (DsRed; red) for 4 h (B), 22 h (C), 46 h (D), and 96 h (E) and decreasing distance between the tumor cells and adipocytes over time is shown in micrometers. Green arrow (X), red arrow (Y), and blue arrow (Z) indicate orientation of the spheroid in 3D space.