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. 2016 May 30;37(7):908–918. doi: 10.1038/aps.2016.30

Figure 6.

Figure 6

Knockdown of the proteasome β1 subunit inhibited the assembly of the 26S proteasome and cellular proteasome activities. (A) Results of the RT-PCR analysis of PSMB6 mRNA levels in negative control cells and PSMB6-knockdown cells. Data are presented as the mean±SEM. n=3 independent experiments. *P<0.05 vs the negative control. (B) Results of the Western blot analysis of proteasome β1 subunit protein levels in negative control cells and PSMB6-knockdown cells. (C) Results of native PAGE analysis of the integral 26S proteasome in the negative control cells and PSMB6-knockdown cells. (D) Results of the enzymatic activity assays in negative control cells and PSMB6-knockdown cells. Data are presented as the mean±SEM. n=3 independent experiments. *P<0.05 vs the negative control.