Antibody responses in mice elicited by immunization with the rAd5-YFV vaccine alone or in combination with rYFV. Mice from different groups (Fig. 4) were also bled at 15 days postimmunization, and an ELISA was performed to examine titers of IgG antibody, its isotypes, and IgA to F1 (A), LcrV (B), and YscF (C), respectively. The P values are in comparison to the indicated groups and based on two-way ANOVA with Tukey's post hoc correction. The asterisks indicate statistical significance compared to the control (Ad5-Empty) mice for IgA levels by using multiple Student's t test with the Holm-Sidak post hoc test correction.