TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Descriptiona	Source or reference(s)
Strains
H10407	Wild-type ETEC strain O78:H11; CFA/1 LT+ ST+ EtpA+	57, 58
jf570	eltAB LT deletion mutant of H10407	59
jf876	lacZYA::Kmr mutant of H10407	59
jf1696	TOP10(pJL017, pJL030) Ampr Cmr	24
TOP10	F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG	Invitrogen
Plasmids
pJL017	etpBA cloned into pBAD/myc-His A, with etpA in frame with myc and 6His coding regions; Ampr	14
pJL030	etpC gene cloned into pACYC184; Cmr	13

^aKm^r, kanamycin resistant; Cm^r, chloramphenicol resistant; Amp^r, ampicillin resistant; Str^r, streptomycin resistant.