Figure 3.

Compound panel of three images of immunofluorescence reactions performed in ipsilateral masseter muscle. A) Longitudinal section of masseter where it is possible to observe an altered, non-rectilinear morphology of fibers and the presence of a lower number of satellite cells PAX-7 positive (red channel) along sarcolemma; nuclei are evidenced by DAPI (blue channel). B) Transversal section of masseter muscle: presence of a very low number of PAX-7 positive cells around sarcolemma (red channel). C) Image of single localization reaction for alpha-sarcoglycan: the protein has not been detected uniformly along the fibers and its fluorescence pattern is almost absent (red channel); satellite cells were positive for alpha-sarcoglycan. Bars: 20 μm