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. 2016 Jul 6;6:29121. doi: 10.1038/srep29121

Figure 2. miR-1 suppressed BDNF expression by mRNA degradation as well as translation repression.

Figure 2

(A) The mRNA expression of BDNF in SCs transfected with miR-1 mimic (miR-1) and miR-1 inhibitor (Anti-miR-1) was analyzed by qRT-PCR. GAPDH was used as an internal control. (B) The protein expression of BDNF in SCs transfected with miR-1 mimic and miR-1 inhibitor was analyzed by Western blot analysis. GAPDH was used as an internal control. (C) Histogram showing relative quantitative comparisons of BDNF protein expressions. (D) The relative luciferase mRNA was analyzed after co-transfection with wild-type or mutant BDNF plus miR-1 mimic (miR-1) or mimic control (miR Con). **p < 0.01, *p < 0.05.