Table 1. Enzyme cloned and expressed in this study.
| Protein Name1 | GH family | % transcript (GH family)2 | Transcript length | CDS3 | Protein length | Cloned region | Genbank Accession number4 | Closest sequenced relative (% amino acid identity, accession number)5 | Closest characterized Relative (% amino acid identity)6 | Reference |
|---|---|---|---|---|---|---|---|---|---|---|
| EG5 | 5 | 7.7–30.9 | 1253 | 1–1251 | 417 | All | KU963308 | Clostridiaceae bacterium AN-C16-KBRB (52%, ADK66823) | Clostridiaceae bacterium AN-C16-KBRB (52%, ADK66823) | This study |
| Cel6A | 6 | 60.4–83.7 | 1283 | 2–1177 | 391 | All | KU963303 | Sorangium cellulosum (45%, KYG0267) | Aspergillus nidulans FGSC A4 (46%, ABF50873.1) | This study |
| Cel48 | 48 | 1–30.3 | 2538 | 87–2342 | 751 | All | KU963307 | Cystobacter fuscus DSM2262 (54%, WP_002632298) | Clostridium cellulolyticum H10 (51%, ACL75108.1) | This study |
| XYL11 | 11 | 21.4–43.7 | 1237 | 3–1010 | 335 | 1–815 | KU963309 | Fibrobacter succinogenes subsp. succinogenes S85 (61%, AKN90969) | Fibrobacter succinogenes subsp. succinogenes S85 (61%, AKN90969) | This study |
| BGL1 | 1 | 27–79 | 2132 | 211–2130 | 639 | 250–2130 | KU963306 | Eucalyptus grandis (39%, XP_010044986) | Phanerochaete chrysosporium K-3 (37%, BAE87009.1) | This study |
| BGL3 | 3 | 4.5–40.9 | 2574 | 101–2380 | 759 | 164–2263 | KU963305 | Mucor circinelloides f. circinelloides 1006PhL (43%, EPB90789) | Rhizomucor miehei CAU432 (43%, AIY32164.1) | This study |
| Swol | NA7 | 21.1–52.3 | 1929 | 92–1822 | 576 | 182–1822 | KU963310 | Aspergillus fumigatus Z5 (56%, KMK55270) | Trichoderma reesei (56%) | This study |
| Bgxg1 | 39 | 58–84 | 1048 | 43–1048 | 335 | 109–1048 | KT997999 | Clostridium saccharoperbutylacetonicum (71%, WP_015392393) | Caldicellulosiruptor saccharolyticus (26%, AAB87373.1) | 12 |
1Proteins used in the final AF cocktails are in bold.
2Values are from Couger et al., 2015, and refer to the percentage of transcripts within a specific GH family that are affiliated with the cloned transcript.
3CDS refers to the region in the mRNA that is transcribed. Numbering refers to the position in the mRNA itself.
4In addition to GenBank accession numbers (public release currently pending), the sequences of all enzymes are provided in the Supplementary document.
5Closest sequenced relative outside the Neocallimastigomycota.
6Closest sequenced and biochemically characterized relative outside the Neocallimastigomycota.
7NA: Not applicable.