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. 2016 Jul 6;6:29154. doi: 10.1038/srep29154

Table 1. Antibodies used for ELISA assays.

Detected Analyte Sample Coating protein Coating Concentration Detection Ab Concentration Depicted in
Murine anti-PR8 IgG Murine sera PR8 virus 500 TCID50/mL in 100 mM NaHCO3 HRP-linked rabbit anti-murine IgG (Sigma; Cat #A9044) 1:10,000 in 1% BSA PBS-T Figure 3c
Ovine IgG in circulation Murine sera Purified donkey anti-sheep IgG (Sigma; Cat # SAB3700721) 5 μg/mL in 100 mM NaHCO3 HRP-linked anti-sheep IgG (Sigma; Cat # A3415) 1:15,000 in 1% BAS PBS-T Figure 4a
Murine anti-ovine IgG Murine sera Ovine pAb 10 μg/mL in 100 mM NaHCO3 HRP-linked rabbit anti-murine IgG (Sigma; Cat # A9044) 1:10,000 in 1% BSA PBS-T Figure 4b
Ovine anti-PR8 IgG Ovine pAb PR8 virus 500 TCID50/mL in 100 mM NaHCO3 HRP-linked anti-sheep IgG (Sigma; Cat # A3415) 1:15,000 in 1% BAS PBS-T Figure 5a
Ovine anti-A/PC IgG Ovine pAb A/PC virus 500 TCID50/mL in 100 mM NaHCO3 HRP-linked anti-sheep IgG (Sigma; Cat # A3415) 1:15,000 in 1% BAS PBS-T Figure 5a

ELISA protocols were developed to assess the levels of murine anti-PR8 IgG, circulating ovine IgG or murine anti-ovine IgG in sera from infected or treated mice, or to assess the ability of ovine IgG to bind to PR8 and A/PC viruses.