Table 1. Antibodies enhance both infection and burst size, but not the specific infectivity of progeny virions.

MOI	Infected cells (%)	Burst size (PFU/cell)	Particles/PFU
1	2.0 ± 0.5	2.8 ± 1.0	255 ± 34
1-ADE	3.7 ± 0.9	8.9 ± 2.4	212 ± 19
10	11.3 ± 2.3	17.2 ± 4.7	268 ± 24

Primary human macrophages were infected at MOI 1 or 10, and MOI 1-ADE. For MOI 1-ADE DENV was pre-incubated for 1 h with 40 ng/mL of antibody #751 A2. The number of infected cells was determined by flow cytometry at 26 hpi. Concurrently, the virus titer was determined by both qRT-PCR (physical particles) and plaque assay (infectious particles). The burst size was calculated by dividing the virus titer by the number of infected cells and the specific infectivity resembles the number of physical particles divided by the number of infectious particles. All values are SEM of duplicates of 4 donors.