Figure 3. Phylogenetic comparison of neuraminidase genes from highly pathogenic avian influenza A (H5N2, H5N8, and H5N1) detected in wild birds from the United States Pacific Flyway.

Sequences were aligned using Clustal W. Evolutionary analyses were conducted in MEGA6 and the evolutionary history was inferred by using the Maximum Likelihood method based on the Hasegawa-Kishino-Yano (HKY) model⁴³,⁴⁴. The tree with the highest log likelihood is shown. Bolded samples were from wild birds sampled in the Pacific Flyway. Analysis of the NA gene involved 57 nucleotide sequences, 43 of which were from samples collected during this surveillance effort.