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. 2016 Jul 6;6:29077. doi: 10.1038/srep29077

Figure 5. Effect of SAP on D76N β2-m amyloid fibril formation in MES-Ca buffer.

Figure 5

(a) Time course of fibril formation monitored by ThT fluorescence in the absence (black diamond) or presence of 1:100 (molar ratio of SAP to D76N β2-m) (red circle), or 1:20 (dark brown circle) SAP. Each point represents the average of three independent incubations. Representative data of three independent experiments are shown. (b,c) ThT fluorescence of each sample at 18 (b) or 75 h (c) in (a). The data are mean ± SD of three independent incubations. Statistical analysis was performed by unpaired Student’s t-test. *P < 0.05, **P < 0.01 vs. control. (d–g) Electron microscopy images of the samples of fibril formation. The sample prepared in the absence (d,f) or presence of 1:20 SAP (e,g) was incubated at 37 °C for 18 h (d,e) or 75 h (f,g). The scale bar represents 0.5 μm (d,e) or 100 nm (f,g). In (g), inset indicates pentameric SAP at the same magnification and red arrows indicate pentameric SAP bound to the surface of amyloid fibrils.