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. 2016 Jul 6;6:29077. doi: 10.1038/srep29077

Figure 7. Interactions of CRP and SAP with fresh and aggregated Aβ(1-40) and D76N β2-m.

Figure 7

(a–f) Binding of Aβ(1-40) (a–c) and D76N β2-m (d–f) to immobilized CRP and SAP. CRP and SAP were immobilized on an ELISA plate and incubated with fresh (f) and aggregated (ag) Aβ(1-40) and D76N β2-m in Tris-EDTA (a,d), Tris-Ca (b,e), or MES-Ca buffer (c,f). The data are mean ± SD of three independent incubations. Representative data of three independent experiments are shown. (g,h) Crosslinking experiments. At 0 h (fresh mixture, f) and at the beginning of the growth phase (aggregated mixture, ag), the reaction mixture containing Aβ(1-40) or D76N β2-m was spiked with 1:20 CRP and SAP, and incubated for 30 min at 37 °C. After BS3 was added to the mixture, SDS-PAGE (top) and western blotting analysis (bottom) were performed. In western blotting analysis, bound Aβ(1-40) and D76N β2-m were detected with anti-human Aβ(1-40) and β2-m antibodies, respectively.