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. 2016 Jul 6;6:28956. doi: 10.1038/srep28956

Figure 2. Effect of repeated curcumin (120 mg/kg, i.p) treatment on the expression of spinal IL-1β in SNI mice.

Figure 2

(A) Western blot showing the expression of Pro-IL-1β and mature IL-1β (mIL-1β) in the spinal cord of repeated DMSO- or curcumin-treated mice on day 7 after SNI or sham surgery. Cropped gels/blots are used in this figure and the immunoblots were obtained from the microgel running in the same experimental conditions. (B) Quantification of the Pro-IL-1β and mIL-1β bands. **p < 0.01 compared with the sham + DMSO group; #p < 0.01 compared with the SNI + DMSO group; one-way ANOVA followed by Bonferroni post hoc test; n = 4 per group. (C) IL-1β and GFAP double staining in the spinal dorsal horn of repeated DMSO- and curcumin-treated mice day 7 after sham and SNI surgery. The arrows indicate the double-labelled cells. (D) Intensity of IL-1β and GFAP staining in the superficial dorsal horn (laminae I–III). *p < 0.05, compared with the Sham + DMSO group; #p < 0.05 compared with the SNI + DMSO group; one-way ANOVA followed by Bonferroni post hoc test; n = 3–4 per group. (F) Number of IL-1β+/GFAP+ double-labelled cells in the superficial dorsal horn (laminae I–III). *p < 0.05, compared with the Sham + DMSO group; #p < 0.05 compared with the SNI + DMSO group; one-way ANOVA followed by Bonferroni post hoc test; n = 3–4 per group. Scale bars = 50 μm.

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