Figure 4. Effect of repeated curcumin treatment on the activation of the spinal NALP1 inflammasome in SNI mice.

(A–D) SNI injury resulted in the increased expression of NALP1, ASC, and Pro- and mature-caspase-1 in the spinal cord on day 7 after SNI. The SNI-induced elevation in NALP1 and Pro- and mature-caspase-1 expression was significantly reduced after repeated curcumin treatment. *p < 0.05 and **p < 0.01; one-way ANOVA followed by Bonferroni post hoc test; n = 4 per group. All of the data are shown as the mean ± SEM. (E) ASC immunoprecipitation from lysates obtained from SNI- or sham-treated mice that received repeated treatment with curcumin (120 mg/kg) or DMSO. Curcumin reversed the SNI-induced aggregation of the NALP1 inflammasome proteins. Cropped gels/blots are used in this figure and the immunoblots were obtained from the microgel running in the same experimental conditions.